ESKIV – Regional Training Courses

Regional Training course on Triatominae and Trypanosomiasis,

and

International Workshop, Nimpe / IRD, Hanoi, (Vietnam)

Epidemiological Status of Kissing Bugs in Vietnam (ESKIV)

Regional Training course on Triatominae and Trypanosomiasis

Day 1 REGIONAL TRAINING COURSE -Introduction to Population studies

8H15-8h30 Introduction Pham Thi Khoa (NIMPE)

8H45-9H30 CJ Schofield (UK) Population dynamics of insects : Laboratory and rearing techniques allowing to study the population growth parameters of each species.

9H45-10H30 CJ Schofield (UK) Population dynamics of insects : Eggs from wild collected females maintained as cohorts in laboratory conditions, with nymphs and adults fed weekly on chickens or

laboratory rodents.

COFFEE

11H00-11H45 JP Dujardin (France) Population structure and reinfestation

LUNCH

14H-16H30 CJ Schofield studies Population dynamics of insects : Cohorts counted weekly to establish rate of recruitment from one stage to the next (following standard ECLAT protocols) in order to derive basic population parameters (Ro, intrinsic rate of increase, intrinsic mortality rates, and fecundity and natality rates) + Practice

DAY 2 REGIONAL TRAINING COURSE – Characterization techniques for insect population studies

8H45-9H30 S Catala (Argentine) Antenal phenotypes: Main physiological and ecological foundations supporting the use of APH to study Triatominae populations.

9H45-10H30 S Catala (Argentine) Antenal phenotypes: Techniques for preparing antennae and counting sensilla under light microscopy.

COFFEE

11H00-11H45 F Panzera (Uruguay) Cytogenetics of Triatominae (Eukaryotic chromosome. Structure, Organization and Types of sequences.)

12H00-12H45 S Catala (Argentine) Statistic analysis and interpretation of APH.

LUNCH

14H-15H30 JP Dujardin Morphometric software (CLIC, R)

15h45-16h30 Truong Xuan Lam IEBR Morphometrics of Triatoma in Vietnam

COFFEE

17h00-17h45 S Catala (Argentine) Animal attractants for Triatominae

Day 3 REGIONAL TRAINING COURSE

8H45-9H30 F Panzera (Uruguay) Holocentric Nature of the Chromosomes of Triatominae: Behavior and Segregation during cell divisions: mitosis and meiosis.

9H45-10H30 F Panzera (Uruguay) Cytogenetic markers used for Taxonomy and population studies in Triatominae.

COFFEE

11H00-12H30 F Panzera (Uruguay) Practice classes – Removal and fixing the gonads of the bugs for the chromosome analysis.

LUNCH

pm – Control of Triatominae

Tools

14H45-15H30 Pham Thi Khoa (NIMPE) Insecticides

Strategies

15H45 – 16H30 C. Schofield (UK) The Southern Cone and CA initiatives

International Workshop, Nimpe / IRD, Hanoi, (Vietnam)

Day 4 INTERNATIONAL SEMINAR, first day

9h00 Opening ceremony (Health Ministry Vietnam, WHO (WPRO), NIMPE, IEBR, French Embassy Hanoï, JICA, IRD)

9h30-10h Chris Schofield LSHTM

Triatominae

10h-10h30 Jean-Pierre Dujardin IRD

Invasive species and emerging diseases

Discussion

COFFEE

10h40-11h Pedro Albajar WHO

Chagas disease outside Latin America

11h20-11h40 Truong Xuan Lam IEBR

T. rubrofasciata : current infestation in Vietnam

11h40-12h Pham Thi Khoa NIMPE

T. rubrofasciata : laboratory rearing and insecticide tests

Discussion

LUNCH

14h00 Field and/or laboratory visit

16h00 Visit of NIMPE and IEBR laboratories, and contacts with students

Day 5 INTERNATIONAL SEMINAR, second day

9h-9h20 Dermatologist Vietnam

Cutaneous lesions and possible allergic responses to insect bites

9h20-9h40 Marc Desquesnes CIRAD

Mechanical transmission and hematophagous bugs

9h40-10h Stephane Herder IRD

Trypanosomes identification techniques

10h-10h20 Serge Morand CNRS

Rodents : migration behaviour in SEA

COFFEE

10h40-12h40

Round Table(s) about strategies and researchto be developed to face the threat of T. rubrofasciata in Vietnam, as well as in South East Asia and other non-endemic countries.

LUNCH

14H20-15H20

Recommandations of the Round Table(s)

COFFEE

15H30-16H

Closing ceremony (Health Ministry Vietnam, WHO (WPRO), NIMPE, IEBR, French Embassy Hanoï, JICA, IRD)